A general stationary phase for analysis
of Cyclodextrins and their derivatives:
A special stationary phase has been developed to cyclodextrin type molecules. The high pure silica was modified with a 4-nitrophenylcarbamide group by a spacer. This stationary phase has been named to CD-Screen, because it is usable generally to screen various types of cyclodextrins and their derivatives based on cavity size and degree of substitution (DS).
The CD-Screen stationary phase is suitable for
– analysis of parent CDs and their degradation products,
– determination of residual parent CDs and degradation products in CD derivatives,
– characterization of component distribution of statistically substituted CD derivatives,
– preparative separation of cyclodextrins and their derivatives.
One of the main advantages of the HPLC method developed on the CD-Screen column is that beta-cyclodextrin (BCD or Betadex) can be quantified in the derivatives (e.g. in hydroxypropyl-BCD, HPBCD) in very low amounts (0.07%). The separation of main component groups gives the possibility to follow batch-to-batch reproducibility. HPBCD samples having different DS can be identified based on their fingerprint chromatogram.
Residual BCD in Hydroxypropyl Betadex